Cytotoxic naphthoquinones fromArnebia densiflora(Nordm.) Ledeb and determining new apoptosis inducers
NATURAL PRODUCT RESEARCH, cilt.34, sa.12, ss.1669-1677, 2020 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 34 Sayı: 12
- Basım Tarihi: 2020
- Doi Numarası: 10.1080/14786419.2018.1525714
- Dergi Adı: NATURAL PRODUCT RESEARCH
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, CINAHL, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
- Sayfa Sayıları: ss.1669-1677
- Anahtar Kelimeler: Alkannin, cytotoxicity, caspase, apoptosis, Arnebia densiflora, Boraginaceae, ARNEBIA, SHIKONIN, CALLUS
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Lokman Hekim Üniversitesi Adresli: Hayır
Özet
In this study, phytochemical composition ofArnebia densiflora(AD) was determined and cytotoxic effects of then-hexane extract and compounds isolated from this species on various cell lines were investigated. By means of serial chromatographic studies, 6 naphthoquinone derivatives were yielded, which are isovalerylalkannin,alpha-methyl-n-butyl alkannin, acetylalkannin,beta-acetoxy isovalerylalkannin, alkannin and a new compound: 4-hydroxy 4-methyl valeryl alkannin. Structures of the isolated compounds were elucidated using UV, IR, 1D-2D NMR, MS and CD methods. Cytotoxic effects of the extract and isolated alkannins were investigated on L929, HeLa, HEp-2 cells. AD and the isolated compounds demonstrated moderate to strong cytotoxic effects (IC(50)range: 4.92-172.35 mu g/ml). The results of DNA fragmentation and caspase-3 activity studies on HeLa cells exhibited that AD and the naphthoquinones isolated from it caused cytotoxicity through induction of apoptosis.