A new combined enzyme-charcoal system formed by enzyme adsorption on charcoal followed by polymer coating


Piskin K., Chang T.

International Journal of Artificial Organs, cilt.3, sa.6, ss.344-347, 1980 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 3 Sayı: 6
  • Basım Tarihi: 1980
  • Doi Numarası: 10.1177/039139888000300610
  • Dergi Adı: International Journal of Artificial Organs
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.344-347
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

A method is described for a new combined enzyme-charcoal system. A model enzyme, urease, was adsorbed on activated charcoal granules which was then coated with ultrathin cellulose nitrate. The assayed activity of this immobilized enzyme ws 28.1% ± 3.12% (mean ± S.D.) of the activity of urease in solution. The enzyme did not leak out after immobilization and worked efficiently. The assayed urease activity increased with greater amounts of urease/gm immobilized enzyme, but reached a plateau after 40 sumner units/gm. Immobilized urease has good storage and operational stabilities at refrigerator, room and body temperatures. In vivo studies show that about 90% conversion ureas in one single pass is possible.