The protective effect of metformin against the noise-induced hearing loss

Kesici G. G., Öcal F. C. A., Gürgen S. G., Erdem Ş. R., Öğüş E., Erbek H. S., ...More

European Archives of Oto-Rhino-Laryngology, vol.275, no.12, pp.2957-2966, 2018 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 275 Issue: 12
  • Publication Date: 2018
  • Doi Number: 10.1007/s00405-018-5161-7
  • Journal Name: European Archives of Oto-Rhino-Laryngology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2957-2966
  • Keywords: Acoustic trauma, Metformin, Noise, Noise-induced hearing loss
  • Lokman Hekim University Affiliated: No


Objective: To test the protective effect of metformin against noise-induced hearing loss. Methods: 24 rats were included in the study. The first group was exposed to noise only, the second group took metformin, the third group was exposed to noise and took metformin, and the fourth group was neither exposed to noise nor took metformin as control group. After measurement of baseline DPOAE and ABR of rats, the metformin group and the metformin + noise group received 300 mg/kg/day metformin via gavage for 10 days. On the 11th day, group 1 and group 3 were exposured to white noise at 105 dB SPL for 15 h. After noise exposure, DPOAE and ABR measurements of all rats were repeated on days 1st, 7th, and 21st. At the end of the study, all animals were sacrificed and cochlear tissues were separated for immunohistochemical assessments. Results: ABR threshold values and DPAOE measurements of groups 1 and 3 were deteriorated on the 1st day after noise, while deterioration in group 1 continued on 7th and 21st days, but normalized on 7th day in group 3. After immune staining, a significant immunoreaction was observed in the noise group, while the reaction in the noise + metformin group was close to the control group. Conclusion: Metformin has a protective effect on noise-induced hearing loss in rats. As a conclusion, it is determined that metformin protects from permanent threshold shift in rats. It can be considered a good alternative for protecting noise-induced hearing loss.