Serum and cord blood-methylated arginine levels in gestational diabetic subjects

Yildirimkaya M. M., ABUŞOĞLU S., YILMAZ S. A., SARAÇLIGİL B., HATAYSAL E. P., Ecer B., ...More

Turkish Journal of Biochemistry, vol.44, no.2, pp.153-160, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 2
  • Publication Date: 2019
  • Doi Number: 10.1515/tjb-2018-0201
  • Journal Name: Turkish Journal of Biochemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.153-160
  • Keywords: Methylated arginines, Gestational diabetes, impaired glucose tolerance, Tandem mass spectrometry, Endothelial dysfunction, NITRIC-OXIDE SYNTHESIS, ASYMMETRIC DIMETHYLARGININE, CIRCULATING CONCENTRATIONS, WOMEN, ASSOCIATION
  • Lokman Hekim University Affiliated: Yes


© 2019 De Gruyter. All rights reserved.Objectives: Micro- and macrovascular endothelial deterioration has been mentioned in diabetic pregnants with critical clinical outcome for the fetus. Our aim was to measure serum and cord blood concentrations of methylated arginines in patients with gestational diabetes and find a relationship with endothelial dysfunction. Materials and methods: Methylated arginines were detected with high performance liquid chromatography mass spectrometry via electrospray ionization positive technique with a chromatographic C18 column. Results: Although gestational diabetes mellitus (GDM) groups' asymmetric dimethylarginine (ADMA) levels were higher compared to control group, this difference was not significant. Control cord blood ADMA and N-monomethylarginine levels were significantly higher than insulin-regulated GDM cord blood ADMA and N-monomethylarginine levels (p = 0.001; p = 0.003, respectively). Diet-regulated GDM group's cord blood N-monomethylarginine was significantly higher than insulin-regulated GDM group's cord blood N-monomethylarginine (p = 0.045). A negative correlation was found between cord blood symmetric dimethylarginine and oral glucose tolerance testing 0h glucose values (r = −0.453, p = 0.002). Conclusions: According to this study's results, methylated arginine levels may not be associated with endothelial deterioration in GDM otherwise with preeclampsia risk.