Akademik Veri Yönetim Sistemi
Trace Elements and Electrocytes, cilt.14, sa.1, ss.13-18, 1997 (SCI-Expanded)
It is generally maintained that combined deficiency of selenium (Se) and vitamin E leads to several diseases in humans as well as to the tissue damage resulting from toxicity. The abnormalities rised from its deficiency can be reversed by the administration of various forms of Se. However, there is a narrow margin between therapeutic and toxic doses. The present study was designed to investigate and compare the effects of dietary Se and vitamin E on the contractility of ileal smooth muscle. Young rabbits of both genders were fed with either a standardized Se and vitamin E adequate diet, a Se and vitamin E deficient diet or a Se rich diet. Deficiency or excess of Se was verified by direct methods (blood Se and glutathione peroxidase analyses) while the acetylcholine-induced contraction amplitude of both experimental group were measured as depressed with respect to the control group (p < 0.05); these depressed amplitudes were also observed when we replaced the bath Tyrode calcium content with 2-fold calcium. Moreover, the mean amplitude of acetylcholine-induced contraction of ileum preparations in both experimental and control groups was in the same range when we replaced bath solution with calcium-free Tyrode solution. Our results can demonstrate the oxidative effects on smooth muscle contractility increased with Se deficiency or excess in diet, and its relationship with calcium. Although it has been pointed out that Se is a highly toxic agent and caution has to be taken, this can be an overriding objection against supplementation of the diet with low doses of Se.