Vaginoplasty using human amniotic membranes: A report of five patients

Avsar A. F., TAŞ E. E., Keskin H. L., Yeğin G. F.

Journal of Reproductive Medicine, vol.61, no.5, pp.483-488, 2016 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 61 Issue: 5
  • Publication Date: 2016
  • Journal Name: Journal of Reproductive Medicine
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.483-488
  • Keywords: 46, XX, 46, XX disorders of sex development, 46, XY disorders of sex development, Amnion, Androgen insensitivity syndrome, Congenital absence of uterus and vagina, Congenital defects, Female, Genital organs, female, Genitalia, Gonadal dysgenesis, Mayer rokitansky kuster hauser syndrome, Mayer-rokitansky-kuster-hauser syndrome, McIndoe vaginoplasty, MRKH anomaly, Mullerian aplasia, Neovagina, Reconstructive surgical procedure, Vaginal agenesis, Vaginoplasty
  • Lokman Hekim University Affiliated: No


© Journal of Reproductive Medicine®, Inc.OBJECTIVE: To present our experiences with an innovative surgical approach for vaginal agenesis that uses amnion as the allograft to create a functional neovagina. STUDY DESIGN: This study involved 5 patients with diagnosed vaginal agenesis. One patient had karyotype 46,XY and was diagnosed with complete androgen insensitivity syndrome, whereas the others had karyotype 46,XX and were diagnosed with Mayer-Rokitansky-Küster-Hauser syndrome. All patients underwent McIndoe vaginoplasty using amnion and were followed up at 2 weeks, 3 months, 6 months, and 12 months postsurgery. Functionality of the neovagina was assessed by Rosen Female Sexual Function Index (FSFI) questionnaire. RESULTS: At the 12-month follow-up 1 patient reported an inability to use the vaginal dilator effectively; for the other 4 patients the mean depth of the neovagina was 9.5 cm and the mean width was 3.5 cm. The mean overall FSFI score was 28.3. CONCLUSION: McIndoe vaginoplasty is a simple, safe, and quick operative method that results in a functional vagina. The amnion is a suitable material to apply to the surface of the neovagina because it is readily available and does not have any associated immune rejection problems or costs.