Akademik Veri Yönetim Sistemi
İstanbul Gelişim Üniversitesi Sağlık Bilimleri Dergisi, cilt.27, sa.2025, ss.824-836, 2025 (TRDizin)
Aim: Mesenchymal stem cells (MSCs) act on cancer cells in the same microenvironment by secreting cytokines, chemokines, exosomes, or by direct contact. The purpose of this study is to analyse the impact of WJ-MSC-derived exosomes on the viability and metabolic activity of lung, pancreatic, and liver cancer cells.
Method: In this study, Wharton's Jelly (WJ)-MSCs-derived exosomes were added to A549, Panc-1, and HepG2 cells as 1-5-10-15 and 20 million particle exosomes. The effects on viability were evaluated by MTT analysis at 48 and 96 hours. The effects on metabolic activity were evaluated by calcein staining at the end of the 96th hour. Cell metabolic activity was calculated using fluorescence intensity.
Results: At the end of the 96th hour, 62% increase in A549 cells, 95% increase in Panc-1 cells and 67% increase in HepG2 cells were detected in the control group. In the group which 20 million exosomes were applied, 36% increase in A549 cells, 43% increase in Panc-1 cells and 31% increase in HepG2 cells were observed. The fluorescence intensity of 20 million exosomes was calculated as: A549: 61%, Panc-1: 64% and HepG2: 68%. Preliminary findings indicate that WJ-MSC-derived exosomes do not increase the viability and metabolic activity of cancer cells when applied to them. It was also shown that the viability and metabolic activity can decrease depending on time and concentration.
Conclusion: WJ-MSC-derived exosomes may be effective on cell viability and metabolic activity in vitro in tumors with high stroma content, and their effects on other mechanisms on tumor cells need to be investigated.