The antioxidant effect of dexmedetomidine on testicular ischemia-reperfusion injury

Creative Commons License

TUĞLU D., Yuvanc E., Yılmaz E., Gencay I. Y., Atasoy P., Kisa U., ...More

Acta Cirurgica Brasileira, vol.30, no.6, pp.414-421, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 6
  • Publication Date: 2015
  • Doi Number: 10.1590/s0102-865020150060000007
  • Journal Name: Acta Cirurgica Brasileira
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.414-421
  • Keywords: Dexmedetomidine, Spermatic Cord Torsion, Antioxidants, Reperfusion Injury, Testis, Rats, INFLAMMATORY RESPONSES, RAT-KIDNEY, TORSION, THERAPY, TISSUE, SHOCK, MICE
  • Lokman Hekim University Affiliated: No

Abstract

© 2015, Sociedade Brasileira para o Desenvolvimento de Pesquisa em Cirurgia. All rights reserved.Purpose: To investigate the protective effect of dexmedetomidine (Dex) on testicular damage induced by ischemia-reperfusion injury in rats. Methods: Sham group underwent left scrotal exploration only (group 1). The ischemia-reperfusion only group underwent left testicular torsion and detorsion (group 2). The ischemia-reperfusion plus Dex group underwent left testicular torsion, received 50 μg/kg Dex (group 3) and 100 μg/kg Dex (group 4) intraperitoneally at minute 180 of ischemia and then underwent detorsion. We determined histopathological findings and performed specific biochemical analyses. Results: Increasing doses of Dex significantly increased TAS, and significantly decreased OSI. Analyzing the antioxidant effects of increasing doses of Dex in torsion and contrlateral testicles: Dex 100 μg/kg statistically significant increased the tissue total antioxidant status (TAS) and oxidative stress index (OSI) when compared with Dex 50 μg/kg but not found significantly change on the tissue total oxidant status (TOS). However, Dex did not significantly improve these histological alterations. Conclusion: The antioxidant effects of dexmedetomidine on testicular ischemia-reperfusion injury in ipsilateral and contrlateral testis, but in the histopathological level, there was no difference statistically according to Johnsen’s scoring system between groups at both sides.