Simultaneous spectrophotometric and liquid chromatographic determination of dextromethorphan hydrobromide, phenylephrine hydrochloride, and carbinoxamine maleate in pharmaceutical preparations


Journal of Analytical Chemistry, vol.67, no.1, pp.56-63, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 67 Issue: 1
  • Publication Date: 2012
  • Doi Number: 10.1134/s1061934812010066
  • Journal Name: Journal of Analytical Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.56-63
  • Keywords: dextromethorphan hydrobromide, phenylephrine hydrochloride, carbinoxamine maleate, spectrometry, liquid chromatography, DRUGS
  • Lokman Hekim University Affiliated: No


Simultaneous determination of dextromethorphan hydrobromide (DEX), phenylephrine hydro- chloride (PHEN), and carbinoxamine maleate (CAR) in pharmaceutical preparations was performed by us- ing liquid chromatograpy (LC) and spectrophotometry. In LC, the separation was achieved on a C18 column and the optimal mobile phase for satisfactory separation in a gradient elution program was found to be ace- tonitrile-sodium perchlorate solution (5 : 95, v/v) initially, then a linear gradient up to 60% acetonitrile in 8 min. In spectrophotometric method, a chemometric technique, principal component regression (PCR), was used. In the method, the absorbance data matrix corresponding to the concentration data matrix was ob- tained by the measurement of absorbances in their zero order spectra by δλ = 1 nm in the 210-300 nm range. Then, the calibration was obtained by using this data matrix for the prediction of unknown concentrations of DEX, PHEN, and CAR in their ternary mixture. The methods proposed were validated and successfully ap- plied to a pharmaceutical preparation, capsule, and the results were compared. © Pleiades Publishing, Ltd., 2012.