The evaluation of thiol/disulphide homeostasis in diabetic nephropathy

Eren M. A., Koyuncu İ., İncebıyık H., Karakaş H., EREL Ö., Sabuncu T.

Diabetes Research and Clinical Practice, vol.148, pp.249-253, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 148
  • Publication Date: 2019
  • Doi Number: 10.1016/j.diabres.2019.01.022
  • Journal Name: Diabetes Research and Clinical Practice
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.249-253
  • Keywords: Diabetes induced nephropathy, Disulphide, Thiol
  • Lokman Hekim University Affiliated: No


Aims: Thiol/disulphide homeostasis plays a critical role in antioxidant defense, and detoxification in body. Although alteration of thiol/disulfide homeostasis had been shown in patients with diabetes, the thiol/disulfide balance in patients with type 2 diabetes and nephropathy is not yet known. Methods: Twenty-six healthy volunteers (group 1), and 17 normal albuminuric (group 2), 24 middle albuminuric (group 3), 20 severe proteinuric (group 4) patients with type 2 diabetes were included. Proteinuria was tested by measuring microalbumin/creatinine ratio in spot urine. Thiol/disulphide homeostasis concentrations were measured using method developed by Erel et al. Results: Mean blood urea and creatinine levels were found to be significantly higher and GFR level was found to be significantly lower in group 4 than in the other groups. Native thiol levels are significantly lower in groups with diabetes than in healthy group and in groups 3 and 4, compared to group 2. Total thiol level was significantly lower in groups 3 and 4 than group 1 and 2. Disulphide/native thiol and disulfide/total thiol ratios were significantly higher in the groups with diabetes than in group 1 and in the group 4 than in the group 2. Conclusion: The level of native and total thiols were found to be decreased significantly with the grade of nephropathy in patients with type 2 diabetes and the balance had been disrupted in favor of disulphide. We suggest that deteriorated thiol/disulphide balance may be one of the important factors in the development or progression of diabetes induced nephropathy.