Purpose Attitudes of individuals towards gender roles may be affected by their experiences such as personality traits. This study is to identify the relationship between university students' personality traits and their attitudes towards gender roles. Design and Methods This cross-sectional was conducted with 301 university students between November 2018 and January 2019. Findings The students who had introverted personality traits had traditional attitudes according to the egalitarian gender role subscale (P < 0.05). Students who had neurotic personality trait had more traditional attitudes according to the female, marriage, traditional, and male gender role subscales (P < 0.05). Practice implications It may be advisable to enable students with an introverted and neurotic personality to develop an egalitarian attitude towards gender roles through women health nursing and psychiatric nursing studies.