Effect of methylphenidate at different doses on new bone formation with rapid maxillary expansion: A micro-CT and histomorphometric study in rats

Dursun S., Sezen Erhamza T., Önder M., Erdoğan Düzcü S.

Orthodontics and Craniofacial Research, vol.25, no.3, pp.384-392, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 3
  • Publication Date: 2022
  • Doi Number: 10.1111/ocr.12547
  • Journal Name: Orthodontics and Craniofacial Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, CINAHL, EMBASE, MEDLINE
  • Page Numbers: pp.384-392
  • Keywords: methylphenidate, new bone formation, rapid maxillary expansion, rat
  • Lokman Hekim University Affiliated: No


Objectives: This study aimed to evaluate the effect of systemically administered methylphenidate hydrochloride (MPH) on new bone formation in premaxillary suture after rapid maxillary expansion (RME). Setting and Sample Population: Thirty-three Wistar rats were divided into four groups: Group 1 (high dose, 30/60 mg/kg MPH), Group 2 (low dose, 4/10 mg/kg MPH), Group 3 (positive control) and Group 4 (negative control). Methods: RME was applied on the 70th day of the study. A 5-day RME period was followed by a 12-day retention period. The experiment was terminated on the 87th day. Micro-CT for radiological evaluation, haematoxylin-eosin and Masson's trichrome staining methods were used for histomorphometric evaluation. Results: Among experimental groups with RME, the lowest number of osteoblasts and capillaries in Group 1 (P <.05). New bone formation, fibrous callus formation, distal osteotomy line, proximal osteotomy union and cortex remodelling were observed to be lower in Group 1 and Group 2 than Group 3 (P <.05). There was a statistically significant difference between Group 4 and each of the other groups (P =.000) in the evaluation of the results for bone mineral density, bone volume, bone volume percentage, trabecular thickness and trabecular number. Conclusions: MPH reduces cellular activity for new bone formation in suture in RME groups. Before performing rapid maxillary expansion in patients using MPH, the use of the drug should be postponed after a multidisciplinary decision process or clinical doses should be lowered.