The effects of adding methylprednisolone to low-potassium dextran solution on preserved ischemic lungs

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Yeginsu A., Ergin M., Özyurt H., ELMAS Ç., GÖKTAŞ G.

Turkish Journal of Medical Sciences, cilt.40, sa.1, ss.39-46, 2010 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 40 Sayı: 1
  • Basım Tarihi: 2010
  • Doi Numarası: 10.3906/sag-0812-5
  • Dergi Adı: Turkish Journal of Medical Sciences
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.39-46
  • Anahtar Kelimeler: Lung transplantation, Methylprednisolone, Organ preservation, Perfadex, Rat
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

Aim: To investigate the effects of adding methylprednisolone to low-potassium dextran solution on cold-preserved ischemic lungs. Materials and methods: The study included 42 rat lung blocks that were divided into 7 groups (n = 6). Lung blocks were preserved in 40 mL of cold Perfadex solution, to which 5, 15, 30, 60, and 120 mg kg-1 (approximately 40, 120, 240, 480, and 960 mg L-1) of methylprednisolone were added. After a 24-h preservation period, the lungs were removed from the solution and examined. Results: As compared to the Perfadex only group, cell death and degeneration were significantly higher in the Perfadex + methylprednisolone groups (P < 0.05). MDA and IL-8 levels were higher in all Perfadex + methylprednisolone groups. Statistically significant differences were observed between some of these groups. Antioxidant enzyme activity was generally higher in the Perfadex + methylprednisolone groups than in the Perfadex only group (P < 0.05). Conclusion: Adding methylprednisolone to low-potassium dextran solution does not have any beneficial effects but has possible deleterious effects in rat lung preservation. © Tübi̇tak.