An in vitro study on the cytotoxicity and genotoxicity of silver sulfide quantum dots coated with meso-2,3-dimercaptosuccinic acid Mezo-2,3-dimerkaptosüksinik asitle kaplanmiş gümüş sülfit kuantum noktalarinin sitotoksisitesi ve genotoksisitesi üzerine bir in vitro çalişma

Creative Commons License

ÖZKAN VARDAR D., Aydin S., Hocaoğlu İ., Yağci Acar H., Başaran N.

Turkish Journal of Pharmaceutical Sciences, vol.16, no.3, pp.282-291, 2019 (ESCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 16 Issue: 3
  • Publication Date: 2019
  • Doi Number: 10.4274/tjps.galenos.2018.85619
  • Journal Name: Turkish Journal of Pharmaceutical Sciences
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.282-291
  • Keywords: Meso-2,3-dimercaptosuccinic acid coated silver sulfide quantum dots, genotoxicity, apoptosis, DNA-DAMAGE, CELL-LINES, PRISTINE NANOPARTICLES, HIGHLY LUMINESCENT, CARBON NANOTUBES, OXIDATIVE STRESS, V79 CELLS, TOXICITY, VIVO, TUMOR
  • Lokman Hekim University Affiliated: No


© Turk J Pharm Sci, Published by Galenos Publishing House.Objectives: Silver sulfide (Ag2S) quantum dots (QDs) are highly promising nanomaterials in bioimaging systems due to their high activities for both imaging and drug/gene delivery. There is insufficient research on the toxicity of Ag2S QDs coated with meso-2,3-dimercaptosuccinic acid (DMSA). In this study, we aimed to determine the cytotoxicity of Ag2S QDs coated with DMSA in Chinese hamster lung fibroblast (V79) cells over a wide range of concentrations (5-2000 μg/mL). Materials and Methods: Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red uptake (NRU) assays. The genotoxic and apoptotic effects of DMSA/Ag2S QDs were also assessed by comet assay and real-time polymerase chain reaction technique, respectively. Results: Cell viability was 54.0±4.8% and 65.7±4.1% at the highest dose (2000 μg/mL) of Ag2S QDs using the MTT and NRU assays, respectively. Although cell viability decreased above 400 μg/mL (MTT assay) and 800 μg/mL (NRU assay), DNA damage was not induced by DMSA/Ag2S QDs at the studied concentrations. The mRNA expression levels of p53, caspase-3, caspase-9, Bax, Bcl-2, and survivin genes were altered in the cells exposed to 500 and 1000 μg/mL DMSA/Ag2S QDs. Conclusion: The cytotoxic effects of DMSA/Ag2S QDs may occur at high doses through the apoptotic pathways. However, DMSA/Ag2S QDs appear to be biocompatible at low doses, making them well suited for cell labeling applications.