An in vitro study on the cytotoxicity and genotoxicity of silver sulfide quantum dots coated with meso-2,3-dimercaptosuccinic acid Mezo-2,3-dimerkaptosüksinik asitle kaplanmiş gümüş sülfit kuantum noktalarinin sitotoksisitesi ve genotoksisitesi üzerine bir in vitro çalişma


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ÖZKAN VARDAR D., Aydin S., Hocaoğlu İ., Yağci Acar H., Başaran N.

Turkish Journal of Pharmaceutical Sciences, vol.16, no.3, pp.282-291, 2019 (ESCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 16 Issue: 3
  • Publication Date: 2019
  • Doi Number: 10.4274/tjps.galenos.2018.85619
  • Journal Name: Turkish Journal of Pharmaceutical Sciences
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.282-291
  • Keywords: Meso-2,3-dimercaptosuccinic acid coated silver sulfide quantum dots, genotoxicity, apoptosis, DNA-DAMAGE, CELL-LINES, PRISTINE NANOPARTICLES, HIGHLY LUMINESCENT, CARBON NANOTUBES, OXIDATIVE STRESS, V79 CELLS, TOXICITY, VIVO, TUMOR
  • Lokman Hekim University Affiliated: No

Abstract

© Turk J Pharm Sci, Published by Galenos Publishing House.Objectives: Silver sulfide (Ag2S) quantum dots (QDs) are highly promising nanomaterials in bioimaging systems due to their high activities for both imaging and drug/gene delivery. There is insufficient research on the toxicity of Ag2S QDs coated with meso-2,3-dimercaptosuccinic acid (DMSA). In this study, we aimed to determine the cytotoxicity of Ag2S QDs coated with DMSA in Chinese hamster lung fibroblast (V79) cells over a wide range of concentrations (5-2000 μg/mL). Materials and Methods: Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red uptake (NRU) assays. The genotoxic and apoptotic effects of DMSA/Ag2S QDs were also assessed by comet assay and real-time polymerase chain reaction technique, respectively. Results: Cell viability was 54.0±4.8% and 65.7±4.1% at the highest dose (2000 μg/mL) of Ag2S QDs using the MTT and NRU assays, respectively. Although cell viability decreased above 400 μg/mL (MTT assay) and 800 μg/mL (NRU assay), DNA damage was not induced by DMSA/Ag2S QDs at the studied concentrations. The mRNA expression levels of p53, caspase-3, caspase-9, Bax, Bcl-2, and survivin genes were altered in the cells exposed to 500 and 1000 μg/mL DMSA/Ag2S QDs. Conclusion: The cytotoxic effects of DMSA/Ag2S QDs may occur at high doses through the apoptotic pathways. However, DMSA/Ag2S QDs appear to be biocompatible at low doses, making them well suited for cell labeling applications.