Multivariate optimization and validation of a CE method for simultaneous analysis of dorzolamide hydrochloride and timolol maleate in ophthalmic solution

PALABIYIK İ. M., ÇAĞLAYAN M. G., ONUR F.

Chromatographia, cilt.73, sa.5-6, ss.541-548, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 73 Sayı: 5-6
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1007/s10337-010-1901-6
  • Dergi Adı: Chromatographia
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.541-548
  • Anahtar Kelimeler: Capillary electrophoresis, Multivariate optimization, Response-surface methodology, Dorzolamide hydrochloride, Timolol maleate, CAPILLARY-ELECTROPHORESIS, EYE DROPS
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

A fast, accurate, precise, and sensitive capillary electrophoretic method has been developed for simultaneous analysis of dorzolamide hydrochloride and timolol maleate in eye drops. Response-surface methodology with a central composite design was used for optimization of buffer pH, applied potential, and temperature. Capillary electrophoresis at 25°C with NaH2PO 4 buffer (pH 6.00, 0.025 M), and an applied potential of 20 kV was found to be suitable, and under these optimum conditions, dorzolamide hydrochloride and timolol maleate were separated in approximately 3 min. This method was validated and successfully applied to a pharmaceutical preparation, ophthalmic solution, and the results were compared with those from a spectrophotometric method described in the literature. © 2011 Springer-Verlag.