Spectrophotometric determination of acetaminophen and chlorzoxazone in tablets by multivariate calibration techniques

DİNÇ E., Ozdemir A., ONUR F., Bǎleanu D., AKSOY H., Yücesoy C.

Revista de Chimie, vol.57, no.4, pp.368-373, 2006 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 57 Issue: 4
  • Publication Date: 2006
  • Journal Name: Revista de Chimie
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.368-373
  • Keywords: multivariate spectral analysis, HPLC method, acetaminophen, chlorzoxazone, pharmaceutical tablet, SPECTRA DERIVATIVE SPECTROPHOTOMETRY, INVERSE LEAST-SQUARES, QUANTITATIVE-ANALYSIS, PSEUDOEPHEDRINE HYDROCHLORIDE, PHARMACEUTICAL FORMULATIONS, BENAZEPRIL HYDROCHLORIDE, LINEAR-REGRESSION, HYDROCHLOROTHIAZIDE, MIXTURE, PARACETAMOL
  • Lokman Hekim University Affiliated: No

Abstract

Three multivariate techniques, Inverse Least Squares (ILS), Principal Component Regression (PCR) and Partial Least Squares (PLS) were applied to the spectrophotometric determination of acetaminophen (AC) and chlorzoxazone (CL) in tablets without prior separation. Stock solutions of acetaminophen and chlorzoxazone were prepared in methanol. Standard solutions were obtained by dilution of stocks with 0.1 M HCl. The multivariate calibrations were built up using training set containing both drugs in different concentration ratios and measuring the absorbances at 14 wavelengths with 5-nm intervals over the wavelength-range of 210-310 nm. Chemometric calculations were performed with MATLAB 70 software. The proposed techniques were applied to a commercial formulation, which contains 300 mgAC and 250 mg CL (n=10). The results were statistically compared with each other, as well as those obtained by the developed HPLC method.