Antibacterial and antioxidant activities of a novel enol ether nor-sesquiterpene isolated from Myrtus nivellei Batt. & Trab.

Demmak R. G., Abdel-Mogib M., Bordage S., Samaillie J., Benssouici C., Bensegueni A., ...More

Fitoterapia, vol.153, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 153
  • Publication Date: 2021
  • Doi Number: 10.1016/j.fitote.2021.104987
  • Journal Name: Fitoterapia
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, CINAHL, EMBASE, International Pharmaceutical Abstracts, MEDLINE, Veterinary Science Database
  • Keywords: Myrtus nivellei, Bioassay guided fractionation, Antibacterial activity, Novel compound, Free-radical scavenging activity, ESSENTIAL OILS, CHEMICAL-COMPOSITION, CLOVE
  • Lokman Hekim University Affiliated: Yes


© 2021Myrtus nivellei is a plant traditionally used to treat diseases including infection of microbial origin. Several M. nivellei Batt. & Trab. extracts (dichloromethane, methanol and ethanol/water) were screened for their activity against 36 microorganisms, including strains resistant to antibiotics. These extracts inhibited on average 15 bacteria strains with minimum inhibition concentrations (MICs) ranging from 0.07 to 1.20 mg/mL. Bioassay guided fractionation was carried out with bioautography on TLC plates using four pathogenic bacteria strains, and following chromatographies (CPC and HPLC) led to the isolation of two novel enol ether nor-cadinane sesquiterpenes from the dichloromethane extract. The major compound (1) showed a strong antibacterial activity. Minimal inhibition concentration and minimal bactericidal concentration (MBC) values were determined against four bacteria: Acinetobacter baumanii, Stenotrophomonas maltophilia, Yersinia pseudotuberculosis and Staphylococcus lugdunensis. The best activity was observed against Acinetobacter baumanii with a MIC value of 9.7 μg/mL. This novel compound was also very active against a Staphylococcus aureus strain resistant to amoxicillin (MIC 19.5 μg/mL). In addition, compound 1 showed a very high antioxidant activity with both DPPH and metal chelate methods.