Propofol attenuates cytokine-mediated upregulation of expression of inducible nitric oxide synthase and apoptosis during regeneration post-partial hepatectomy


Yagmurdur H., Binnetoglu K., ASTARCI H. M., Yagmurdur M. C.

Acta Cirurgica Brasileira, vol.32, no.5, pp.396-406, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 5
  • Publication Date: 2017
  • Doi Number: 10.1590/s0102-865020170050000009
  • Journal Name: Acta Cirurgica Brasileira
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.396-406
  • Keywords: Propofol, Ketamine, Liver Regeneration, Hepatectomy, Anesthesia, Intravenous, Rats, ISCHEMIA-REPERFUSION INJURY, PORTAL HYPERTENSIVE-RATS, LIVER-REGENERATION, SHEAR-STRESS, IN-VITRO, CELL, KETAMINE, RELEASE, LIPOPOLYSACCHARIDE, PROLIFERATION
  • Lokman Hekim University Affiliated: No

Abstract

© 2017, Sociedade Brasileira para o Desenvolvimento de Pesquisa em Cirurgia. All rights reserved.Purpose: To determine the effects of propofol and ketamine anesthesia on liver regeneration in rats after partial hepatectomy (PHT). Methods: Male Wistar albino rats were assigned randomly to four groups of 10. Anesthesia was induced and maintained with propofol in groups 1 and 2, and with ketamine in groups 3 and 4. PHT was undertaken in groups 1 and 3. Rats in groups 2 and 4 (control groups) underwent an identical surgical procedure, but without PHT. At postoperative day-5, rats were killed. Regenerated liver was removed, weighed, and evaluated (by immunohistochemical means) for expression of inducible nitric oxide synthase (iNOS), endothelial NOS (eNOS), apoptosis protease-activating factor (APAF)-1, and proliferating cell nuclear antigen (PCNA). Also, blood samples were collected for measurement of levels of tumor necrosis factor (TNF)-α and interleukin (IL)-6. Results: Between groups 2 and 4, there were no differences in tissue levels of iNOS, eNOS, and APAF-1 or plasma levels of TNF-α and IL-6. eNOS expression was similar in group 1 and group 3. Expression of iNOS and APAF-1 was mild-to-moderate in group 1, but significantly higher in group 3. Groups 1 and 3 showed an increase in PCNA expression, but expression in both groups was comparable. Plasma levels of TNF-α and IL-6 increased to a lesser degree in group 1 than in group 3. Conclusion: Propofol, as an anesthetic agent, may attenuate cytokine-mediated upregulation of iNOS expression and apoptosis in an animal model of liver regeneration after partial hepatectomy.