Performance of different screening methods for the determination of urinary glycosaminoclycans

Afyoncu E., Yilmaz G., Yilmaz F. M., YÜCEL D.

Clinical Chemistry and Laboratory Medicine, cilt.51, sa.2, ss.347-350, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 51 Sayı: 2
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1515/cclm-2012-0471
  • Dergi Adı: Clinical Chemistry and Laboratory Medicine
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.347-350
  • Anahtar Kelimeler: extracellular matrix, glycosaminoglycans, method comparison, proteoglycans, urine, GLYCOSAMINOGLYCAN EXCRETION, DIMETHYLMETHYLENE BLUE, MUCOPOLYSACCHARIDOSES
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

Background: The study aim was to compare the performance of three different methods used for determining urinary glycosaminoglycans (GAG) levels in spot and 24-h urine samples. Methods: Performance characteristics were studied for cetylpyridinium chloride (CPC), and manual and automated dimethylmethylene blue (DMB) methods. Results: For automated DMB method, within-run precisions were 9.10% and 1.98%, and between-day precisions were 13.0% and 5.81% in low- and high-urine pools, respectively. The method was linear up to 100 mg/L of GAG concentration. The detection limit of the method was 0.71 mg/L. Mean recovery was 95.7%. Conclusions: The automated DMB method was found to give better performance characteristics than cetylpyridinium chloride (CPC) and manual DMB methods. It is a fast, cheap, simple and reliable method and can be applied in many diseases in which GAG is used as a screening test. © 2013 by Walter de Gruyter Berlin Boston.