Impact of calcium propionate on cellular behavior in A549 and DMS114 lung cancer cell lines

Okyay T. M., Güler E. M., Kale E., GÜLTEKİN F., Koldas M.

Turkish Journal of Biochemistry, vol.49, no.5, pp.666-673, 2024 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 49 Issue: 5
  • Publication Date: 2024
  • Doi Number: 10.1515/tjb-2024-0054
  • Journal Name: Turkish Journal of Biochemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Food Science & Technology Abstracts, Directory of Open Access Journals
  • Page Numbers: pp.666-673
  • Keywords: calcium propionate, cancer, cell viability, food additive, lung cancer
  • Lokman Hekim University Affiliated: Yes

Abstract

To investigate the cellular and apoptotic effects of food additive calcium propionate by in vitro methods. Cell viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, intracellular Glutathione (GSH) by luminometric method, mitochondrial membrane potential (MMP) by fluorometry, apoptosis by dual Acridine Orange/Ethidium Bromide (AO/EB) fluorescent staining were studied in lung cells. It has been observed that calcium propionate reduced GSH and MMP levels significantly while increased cytotoxicity, apoptosis, and reactive oxygen species (ROS) levels in both A549 and DMS114 cancer cells in a dose-dependent manner. Cytotoxic effects of calcium propionate were more pronounced in cancer cells compared to healthy cells, suggesting its potential suitability as a chemo-preventive agent.