Macrolide resistance determinants of group A streptococci in Ankara, Turkey

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Acikgoz Z. C., Gocer S., Tuncer S.

Journal of Antimicrobial Chemotherapy, vol.52, no.1, pp.110-112, 2003 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 52 Issue: 1
  • Publication Date: 2003
  • Doi Number: 10.1093/jac/dkg300
  • Journal Name: Journal of Antimicrobial Chemotherapy
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.110-112
  • Keywords: Streptococcus pyogenes, macrolide-lincosamide-streptogramin B resistance, MLSB, ERYTHROMYCIN-RESISTANT, PYOGENES
  • Lokman Hekim University Affiliated: No


Objectives: Macrolide-lincosamide-streptogramin B (MLSB) resistance determinants of group A streptococci (GAS) in Ankara, Turkey, were defined for the first time. Isolates and methods: A total of 1355 GAS isolates, collected from three different regions of Ankara, were screened for erythromycin resistance. Resistance phenotypes were determined by a triple-disc test, and the gene determinants responsible were determined by PCR. MICs of erythromycin, clindamycin and spiramycin were measured for the resistant isolates, and susceptibility rates to some further antibiotics were determined. Results: Thirty-six isolates (2.6%) were resistant to erythromycin. Of these, 17 (47.2%) expressed macrolide-restricted resistance (M phenotype), while the remainder expressed inducible (16 isolates, 44.4%) or constitutive (three isolates, 8.3%) MLSB resistance. All isolates of the M phenotype harboured the mefA) gene. Of non-M isolates, 14 harboured erm(A) subclass erm(TR) and five had erm(B) genes. There was a significant relationship between tetracycline resistance and the inducible phenotype (P < 0.05). Macrolide resistance was significantly higher in adults (P < 0.05), and increased more than two-fold in 2002 compared with 2001 (P < 0.05). Conclusion: The prevalence of macrolide resistance in GAS is low in Ankara; therefore, routine anti-microbial susceptibility testing against these agents seems unwarranted.