Peroxynitrite in reperfusion arrhythmias and its whole blood chemiluminescence results

Tecder-Ünal M., Kanzýk Ý.

Pharmacological Research, vol.49, no.1, pp.7-16, 2004 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Abstract
  • Volume: 49 Issue: 1
  • Publication Date: 2004
  • Doi Number: 10.1016/j.phrs.2003.07.001
  • Journal Name: Pharmacological Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.7-16
  • Keywords: peroxynitrite, reperfusion, arrhythmias, chemiluminescence, whole blood, NITRIC-OXIDE, MYOCARDIAL-ISCHEMIA, INJURY, SUPEROXIDE, OXYGEN, HEART, RATS
  • Lokman Hekim University Affiliated: No


The aims of the present study were to investigate the effects of exogenous peroxynitrite (ONOO-) on reperfusion arrhythmias in anaesthetized rats, and to detect endogenous and exogenous ONOO--induced chemiluminescence (CL) signals in rat whole blood, which was collected during baseline and in the first minute of reperfusion. ONOO- infusion in ischemia/reperfusion (I/R) applied groups caused significant decreases in mean arterial pressure (MAP) and heart rate (HR). Ventricular fibrillation (VF) incidences in the vehicle, ONOO-, and dec-ONOO- infused groups were 63, 100, and 20%, respectively. In control group CL signal was 136±34mV during the resting period and was increased to 336±57mV with reperfusion. Also, the effects of SOD+CAT, L-NAME and urate were investigated. Ventricular tachycardia (VT) incidence was decreased significantly in SOD+CAT and urate; VF incidence was decreased significantly in SOD+CAT applied groups. CL signals were inhibited with SOD+CAT, L-NAME, and urate. Exogenous ONOO- infusion during I/R was also investigated. CL signal in exogenously ONOO- infused group is increased 423% during reperfusion. Only urate infused group VF incidence was decreased significantly. CL signals of ONOO- infused groups were inhibited by SOD+CAT, L-NAME, and urate. Based on the results of the current study, ONOO- seems to be one of the key mediators of reperfusion arrhythmias in anaesthetized rats. © 2003 Elsevier Ltd. All rights reserved.