Superoxide dismutase and glutathione peroxidase in erythrocytes of patients with iron deficiency anemia: Effects of different treatment modalities

Isler M., Delibas N., Guclu M., Gultekin F., Sutcu R., Bahceci M., ...Daha Fazla

Croatian Medical Journal, cilt.43, sa.1, ss.16-19, 2002 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43 Sayı: 1
  • Basım Tarihi: 2002
  • Dergi Adı: Croatian Medical Journal
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.16-19
  • Anahtar Kelimeler: anemia, iron deficiency, glutathione peroxidase, iron, superoxide dismutase, vitamin E, VITRO OXIDATIVE STRESS, FREE-RADICALS, VITAMIN-E, SUSCEPTIBILITY, HEMOLYSIS, CELLS
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

Aim. To test whether the activities of erythrocyte superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) can be affected by oral iron (OI) treatment, parenteral iron (PI) treatment, and parenteral iron treatment with vitamin E supplementation (PIE) in iron deficiency anemia. Methods. Twenty-eight patients with iron deficiency anemia and 18 healthy controls were included in the study. Anemic patients were systematically allocated into 3 treatment groups. The first group (n = 8) received OI, the second group (n = 10) PI, and the third group (n = 10) PIE. SOD and GSH-Px activities were determined using commercial kits. Results. Before the treatment, SOD activity was significantly lower in anemic patients than in the control group (Kruskal-Wallis test, p < 0.05). After the treatment, SOD activity significantly increased in all three patient groups and reached the values found in the control group (Wicoxon signed-rank test, p = 0.017 for OI, p = 0.047 for PI, and p = 0.037 for PIE group). Before the treatment, GSH-Px activities in anemic patients were similar to that of control group (Kruskal-Wallis test, p > 0.05). Although there was no significant decrease in GSH-Px activity after OI treatment, both PI and PIE treatments significantly decreased GSH-Px activity (Wilcoxon signed-ranks test, p = 0.007 for PI and p = 0.005 for PIE). PIE was more effective than PI treatment in maintaining GSH-PX activity. Conclusion. Oral iron treatment improved the iron deficiency anemia and recovered antioxidant defense system by increasing SOD activity and maintaining GSH-Px activity at normal level. When parenteral iron treatment is inevitable, vitamin E supplementation together with PI treatment may be effective in partially restoring the antioxidant status in this type of anemia.