Wound Tissue Malondialdehyde and Glutathione Levels in Leptin Treated Healthy and Diabetic Rats Leptin uygulanan saǧliklí ve diyabetik siçanlarda yara Dokusu malondialdehit ve glutatyon düzeyleri


DİNCER S., Gülen Ş.

Erciyes Tip Dergisi, cilt.32, sa.3, ss.161-166, 2010 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 3
  • Basım Tarihi: 2010
  • Dergi Adı: Erciyes Tip Dergisi
  • Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.161-166
  • Anahtar Kelimeler: Glutathione, Leptin, Lipid peroxidation, Streptozotocin, Wound healing
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

Objectives: We aimed to investigate the effects of leptin on wound tissue malondialdehyde and reduced glutathione levels in Streptozotocin-induced diabetic and healthy rats. Patients and Methods: Experiments were performed on 28 male Wistar rats. Animals were divided as healthy control (n = 14) and Streptozotocin -induced diabetic (n = 14) rats. Diabetes was induced by injection of Streptozotocin (intraperitoneally, 55 mg/kg bw). On the 7th day after administration of Streptozotocin, six full-thickness excisional wounds were made under anesthesia in all animals. Then, both healthy and diabetic animals were divided into systemic leptin (intraperitoneally; 0.1mg/kg/day, for 5 days) and vehicle (Phosphate buffered saline (PBS)) subgroups. For topical administrations, wounds of all rats were covered with 5 μg leptin (in 20 μl PBS, for 5 days- right side wounds) and vehicle (left side wounds). At the end of these administrations, the animals were sacrificed and MDA and GSH analysis were made on wound tissues. Results: High MDA and low GSH levels were observed in diabetic control wounds than in healthy control wounds. Topical treatment of normal wounds and systemic and topical co-treatment of diabetic wounds with leptin decreased malondialdehyde levels of wound tissue. Conclusion: Wound tissue oxidative damage might diminish via leptin in healthy and diabetic rats.