Akademik Veri Yönetim Sistemi
49th FEBS Congress, İstanbul, Türkiye, 6 - 09 Temmuz 2025, sa.35012, ss.345, (Özet Bildiri)
Sulfamethoxazole (SMX) is a costeffective sulfonamidederived antimicrobial drug used for both prevention and treatment of many bacterial infections for indications
such as urinary tract infection, acute otitis media, treatment of shigellosis, approved by the FDA in combination with trimethoprim. Human Umbilical Vein Endothelial
Cells (HUVECs) are derived from human umbilical cord and widely used in angiogenesis studies. Endothelial cells make it possible for newly formed tissues to grow
and survive due to their special role in providing blood flow to tissues. Disruption of this condition negatively affects healing. The HUVEC is useful to study the effects of new drugs on human endothelium. In this study, cell viability and migration ability of the HUVEC cell line were examined in vitro following the administration of different concentrations of SMX followed by PCR assays. In order to examine the effects on cell viability, the HUVEC cell line was incubated with SMX at 1μM, 10μM,
50μM, 100μM, 250μM, 500μM and 1 mM doses for 24 hours. At the end of the period, MTT assay was performed to determine the effect on viability. An increase
in viability ranging from 3% to 10% was observed at all doses compared to the control group. The migration ability of HUVEC cells after SMX administration was
examined using 1, 50 and 100μM doses. After 24 hours, while 22% closure was observed in the control, approximately 38% (1μM), 30% (50μM), and 32%
(100μM) closure was observed in study groups. The evaluation of the effect of SMX (50μM and 100μM) on COL1A1 gene expression resulted in downregulation
of COL1A1 gene expression. This study was conducted to determine the effects of SMX on endothelial cells, which play an important role in wound healing process, in addition to its routine indications in clinic. It is important to elucidate the antimicrobial effects of SMX as well as its effects on cells in the same environment in order to evaluate the safety profile of the drug.