Antioxidant Effects of Some Asperula L. (Rubiaceae) Species and Their Inhibitory Potential on Lipoxygenase Enzyme

Büyükyıldırım T., Kahraman A., Şenol Deniz F. S., Erdoğan Orhan .

IV. Bio.Natural Conference, Lisbon, Portekiz, 14 - 16 Temmuz 2025, ss.131, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Lisbon
  • Basıldığı Ülke: Portekiz
  • Sayfa Sayıları: ss.131
  • Lokman Hekim Üniversitesi Adresli: Evet

Özet

The genus Asperula L. is in the Rubiaceae family and has about 200 species. The

flowering shoots of some Asperula species are used in folk medicine as a

diuretic and tonic and against diarrhea. The presence of iridoid glycosides,

cardenolides, flavonoids and anthraquinone glycosides have been reported in

Asperula species.

In this study, a total of 21 extracts were prepared, including dichloromethane,

ethylacetate and ethanolic extracts of some Asperula species collected from

Türkiye (A. cymulosa (AMU), A. fidanii (AFI), A. involucrata (AIN), A. kotschyana

(AKO), A. prostrata (APR), A. suavis (ASU), and A. taurina (ATA)). All of extracts were evaluated at a stock concentration of 2 mg/mL for antioxidant (cupric ion reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), nitric oxide (NO) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical

scavenging activity, iron chelation activity) and lipoxygenase (LOX) enzyme

inhibition activity by spectrophotometry. When the results were evaluated, AFI

ethanol extract was found to have the highest activity in CUPRAC (0.928 ± 0.089 at 495 nm), FRAP (0.547 ± 0.020 at 700 nm), and DPPH (33.55% ± 4.07) radical

scavenging antioxidant activity determinations. Dichloromethane extract of

ASU had the highest antioxidant activity in NO radical scavenging activity (79.24% ± 1.92) and metal chelation activity (81.23% ± 1.52). APR ethylacetate

extract (81.16% ± 3.20) had the highest inhibitory activity against LOX enzyme

inhibition among the extracts.

As a conclusion, there are not many biological activity studies on Asperula

species. Within the scope of this study, it has contributed to the literature in the

evaluation of the antioxidant and anti-inflammatory effects of extracts

prepared in different polarities of Asperula species and it has been observed

that they have remarkable biological activities.

Acknowledgements: Support was provided by TUBITAK project number 123Z351.