Akademik Veri Yönetim Sistemi
Gazi Medical Journal, cilt.24, sa.4, 2013 (ESCI)
Objective: When the body temperature reaches the value of 41°C or higher hyperthermia occurs and this may lead to the destruction of heat regulation mechanisms. The rise of temperature within the fallopian tubes (tuba uterina) leads to apoptosis of ciliated cells, development of oxidative stress and early embryonic death. Superoxide dismutase (SOD) is an antioxidant believed to be able to prevent oxidative stress and reverse apoptotic process by removing free oxygen radicals. This study aims to immunohistochemically detect protective effects of heat stress caused by hyperthermia, SOD used before the stress on tuba uterina by using apoptotic and oxidative stress markers. Methods: 18 Wistar-albino female rats used within the study were separated into 3 groups, with 6 rats in each group. Test subjects within the control group were held for 20 minutes in the pool of 22°C and dissected 24 hours later. Subjects of second and third groups were held for 20 minutes in the pool of 42°C, and respectively dissected 30 minutes and 24 hours later, and their tuba uterina tissues removed. Groups where hyperthermia was applied were injected with NaCl+Catalase+SOD one hour before. In order to determine apoptosis caused by hyperthermia, removed tissues were subjected to indirect immunohistochemical method with HSP-70 primary antibodies to detect impact on Caspase-3, Caspase-8 and Caspase-9 protein structures. Results: Through conducted assessments it was determined that Caspase 3 retention was more evident. It was tracked that parallel to hyperthermia application immunoreactivity increased especially in epithelia, cell cytoplasm and cilias. It was detected that Caspase 3 retention does not increase significantly with hyperthermia. It was observed that for all caspases SOD application reduces retention parallel to time. It was identified that HSP-70 retention occurs generally in epithelia cells on cytoplasm level moderately, and with SOD application retention increases parallel to time. Conclusion: It was concluded that with the activation of caspases, the central components of apoptotic program, especially in epithelia cells hyperthermia starts apoptosis from the external pathway. It was deduced that SOD+Catalase application represses apoptosis parallel to time, which probably happens due to the increased HSP-70's protective effect. © Copyright 2013 by Gazi University Medical Faculty.