Akademik Veri Yönetim Sistemi
Turkish Journal of Biochemistry, cilt.34, sa.1, ss.39-43, 2009 (SCI-Expanded)
Objectives: This study aims at analyzing the antiproliferative effects of three novel benzimidazole retinoids on HL-60 cells and their DNA binding. Methods: Benzimidazole retinoids were synthesized as reported previously. Cells were grown in medium supplemented with 10% fetal calf serum and antibiotics. All-trans retinoic acid, 9-cis retinoic acid and retinoids at different doses were added to cells. Cell proliferation is determined by measuring the amount of DNA using 4, 6-diamidino 2 phenyl indole staining. Plasmid was grown in bacteria and isolated. Retinoids, ATRA, ethidium bromide and 4, 6-diamidino 2-phenyl indole were added to the plasmids at a ratio of 10-5 M to 5μg DNA. These were then electrophoresed on agarose gel and stained with ethidium bromide. Octameric oligonucleotides without self complementarity consisting of adenine and thymine were incubated with retinoids and run in polyacrylamide gel electrophoresis then visualized by silver staining. Results: Benzimidazole retinoids have suppressed cell growth in a dose dependent manner. Cell growth inhibition was not significantly different to those induced by all-trans retinoic acid and 9-cis retinoic acid. Retinoids were bound to plasmid DNA in a similar mode with 4, 6-diamidino 2 phenyl indole. Conclusion: These novel retinoids showing significant amount of antiproliferative effects on HL-60 may be potential anticancer agents and their DNA binding potential may point out their further efficiency in interfering the growth related processes in these cells. Their specific actions however remain to be elucidated.