A method for high-purity isolation of neutrophil granulocytes for functional cell migration assays


Avci E., Akkaya-Ulum Y. Z. , YÖYEN ERMİŞ D., ESENDAĞLI G., PEYNİRCİOĞLU B.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, vol.44, no.6, pp.810-821, 2019 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 6
  • Publication Date: 2019
  • Doi Number: 10.1515/tjb-2019-0089
  • Title of Journal : TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Page Numbers: pp.810-821
  • Keywords: Neutrophil isolation, Cell migration, Purity of neutrophils, In vitro cell migration assay, Autoinflammatory diseases, FAMILIAL MEDITERRANEAN FEVER, EX-VIVO, PRESERVATION, EXPRESSION, APOPTOSIS

Abstract

Background: Neutrophil-mediated killing of pathogens is one of the most significant functions of the primary defense of the host. Neutrophil activity and migration play a key role in inflammatory conditions. To gain insights into the interactions between neutrophils and neutrophil migration-related disorders, a large number of sophisticated methods have been developed. The technical limitations of isolating highly purified neutrophil populations, minimizing both cell death and activation during the isolation process, and the short lifespan of neutrophils present challenges for studying specific functions of neutrophils in vitro. In this study, we aimed to evaluate a separation medium-based density gradient method to obtain highly purified neutrophil populations and combined this protocol with a model for studying neutrophil migration in-vitro.