Determination of Artemisinin in Selected Artemisia L. Species of Turkey by Reversed Phase HPLC

Erdemoglu N., ERDOĞAN ORHAN İ., KARTAL M., Adiguzel N., Bani B.

RECORDS OF NATURAL PRODUCTS, cilt.1, ss.36-43, 2007 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1
  • Basım Tarihi: 2007
  • Dergi Adı: RECORDS OF NATURAL PRODUCTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, ABI/INFORM, CAB Abstracts, EMBASE, Veterinary Science Database, Directory of Open Access Journals, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.36-43
  • Anahtar Kelimeler: Artemisia L., Artemisia annua L., Compositae, HPLC, PERFORMANCE LIQUID-CHROMATOGRAPHY, ANNUA L., GAS-CHROMATOGRAPHY, DERIVATIVES, EXTRACTION, ACID
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

Artemisinin (quinghaosu) is a natural compound, isolated from Artemisia annua L. (Compositae), of current interest in treatment of drug-resistant malaria. Aim of the current study was to hit upon novel artemisinin sources as alternative to A. annua. Therefore, ten species of the genus Artemisia (A. santonicum L., A. taurica Willd., A. spicigera K. Koch, A. herba-alba Asso, A. haussknechtii Boiss., A. campestris L., A. araratica Krasch., A. armeniaca Lam., A. austriaca Jacq., and A. abrotanum L.) collected from different localities throughout Turkey were analyzed with respect to artemisinin amount using a reliable and fast Reversed Phase-High Performance Liquid Chromatography (RP-HPLC) analysis technique. HPLC conditions used for determination of the artemisinin content were established as follows; ACE-5 C18 column (250 x 4.6 mm, 5 mu m) was employed with the mobile phase of formic acid (% 0.2 v/v): acetonitrile (50:50 v/v) mixture at the flow rate of 1 mL/min. The good linearity of artemisinin was found within the range of 0.1-100 mu g/mL (r(2)=0.99908). However, our study showed that artemisinin was not detected in all investigated Artemisia species of Turkish origin.