Akademik Veri Yönetim Sistemi
Turkish Journal of Biochemistry, cilt.35, sa.3, ss.230-235, 2010 (SCI-Expanded)
Purpose: We aimed to show the error sources due to discrepancy between strip analysis by Urisys 2400 and microscopic analysis by UF 100 and to detect error frequency. Materials and methods: A total of 1968 samples were analyzed by Urisys 2400/Sysmex UF 100 automated urinalysis system and 326 discrepant samples were also analyzed by light microscopy as a "gold standard". Results were classified into three groups as mismatch of erythrocytes, leukocytes and renal cast. Results: Average rate of discrepancy detected between chemical and microscopic analysis of the system was 14.2%. Among 326 discrepant samples, 259 (79.4%) had erythro-cyte mismatch, 203 (50%) had leukocyte mismatch, and 79 (24%) had pathological cast mismatch. As compared with the manual microscopy, false positivity was 15.3% and false negativity was 8.9% for erythrocyte; false positivity was 27.3% and false negativity was 2.8% for leukocyte; and false positivity for renal cast was 23.3%. Conclusions: The reason of the false negativity for erythrocyte could be lysis or structural changes due to loses of erythrocyte content in samples with lower specific gravity. Urine samples crowded by mucus, epithelial cells, and leukocytes result in falsely positive pathological cast. In conclusion, if there is any discrepancy between the results of chemical and microscopic analyses the samples should be examined with manual microscopy. © TurkJBiochem.com.