Comparison of creatinine measurement methods developed for "true" creatinine gerçek kreatinin ölçümü i̇çin geliştirilen kreatinin ölçüm yöntemlerinin karşılaştırılması

Aydinbelge B., Kilinç A. S., Diri B., uranay M., Saydam G., YÜCEL D.

Turkish Journal of Biochemistry, cilt.36, sa.4, ss.356-361, 2011 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 4
  • Basım Tarihi: 2011
  • Dergi Adı: Turkish Journal of Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.356-361
  • Anahtar Kelimeler: Chronic kidney disease, Creatinine, Glomerular filtration rate, Method comparison, Standardization
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

Objective: Commonly used creatinine methods are based on Jaffe reaction. Jaffe reaction is interfered by various interferents. Our aim was to improve and automate "true" creatini-ne measurement methods based on Jaffe reaction and to compare the performance of these methods.Methods: Sera were collected from patients with chronic kidney disease (CKD, n = 102) and healthy controls (n = 37). Creatinine was measured by enzymatic method, kinetic method based on Jaffe reaction, acidification method, and "buffered methods" [(pH 7.8, Buffered1) and (pH 9.65, Buffered2)] and a method based on creatinine adsorption to Fuller's earth. Enzymatic method was considered as a reference in method comparison.Results: All the methods used gave lower creatinine concentrations than Jaffe kinetic method. Relationships between enzymatic method (x) and the others (y) were as following: Kinetic method: 0.24 + 0.98x; acidification method: 0.27 + 0.87x; Buffered1: 0.22 + 0.91x; Buffe-red2: 0.10 + 0.96x and adsorption method: 0.10 + 0.96x.Conclusions: All methods can be automated except adsorption method. Performance of the methods is comparable with Jaffe kinetic and enzymatic methods. Among the methods, Buf-fered2 is the best compatible with enzymatic method. It is cheaper, suitable for routine studies and it can be automated. © TurkJBiochem.com.