Akademik Veri Yönetim Sistemi
3rd International Gazi Pharma Symposium Series, Ankara, Türkiye, 8 - 10 Eylül 2021, ss.166
The genera Geranium and Erodium belonging to the Geraniaceae family have been reported
to have various ethnopharmacological uses worldwide. In this study, the ethanol extracts
obtained from Geranium and Erodium species were screened for their DPPH radical
scavenging and acetylcholinesterase enzyme inhibitory activity, which are associated with
the pathophysiology of Alzheimer's disease.Geranium and Erodium species were collected
throughout Turkey. The antioxidant activity of the extracts prepared with 96% ethanol was
investigated by DPPH radical scavenging method and their in vitro inhibitory effect on AChE
were determined using ELISA microtiter assays. 24 extracts out of 47 extracts showed
concentration-dependent inhibitory effect towards AChE over 50% inhibition. The highest
AChE inhibitory activity at 2000 µg/mL was found in G.macrostylum Boiss. collected from
Antalya 87.90 ± 2.15% (IC50= 4.73± 2.96 µg/mL). The inhibitory activity of galanthamine at
1000 µg/mL, used as the reference drug, was found 97.11±1.26% (IC50= 0.68 ± 0.05
µg/mL). All of extracts exhibited excellent antioxidant activity. The highest antioxidant
activity at 2000 µg/mL was found in G.purpureum Vill. collected from Antalya (85.80 ±
0.26%). The radical scavenging activity of quercetin used as reference to compare in the
antioxidant activity was found 85.51 ± 0.17 at 1000 µg/mL.Our findings revealed that
Geranium and Erodium species screened in this study display high radical scavenging
activity against DPPH and remarkable inhibitory activity against AChE.The phytochemical
analysis of the active extracts to elucidate the compounds responsible for the mentioned
activities is in progress in our laboratory