Evaluation of acetylcholinesterase inhibitory and DPPH radical scavenging activity of Geranium and Erodium taxa from Turkey


Abacı N., Şenol Deniz F. S., Kahraman A., Erdoğan Orhan İ.

3rd International Gazi Pharma Symposium Series, Ankara, Türkiye, 8 - 10 Eylül 2021, ss.166

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.166
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

The genera Geranium and Erodium belonging to the Geraniaceae family have been reported to have various ethnopharmacological uses worldwide. In this study, the ethanol extracts obtained from Geranium and Erodium species were screened for their DPPH radical scavenging and acetylcholinesterase enzyme inhibitory activity, which are associated with the pathophysiology of Alzheimer's disease.Geranium and Erodium species were collected throughout Turkey. The antioxidant activity of the extracts prepared with 96% ethanol was investigated by DPPH radical scavenging method and their in vitro inhibitory effect on AChE were determined using ELISA microtiter assays. 24 extracts out of 47 extracts showed concentration-dependent inhibitory effect towards AChE over 50% inhibition. The highest AChE inhibitory activity at 2000 µg/mL was found in G.macrostylum Boiss. collected from Antalya 87.90 ± 2.15% (IC50= 4.73± 2.96 µg/mL). The inhibitory activity of galanthamine at 1000 µg/mL, used as the reference drug, was found 97.11±1.26% (IC50= 0.68 ± 0.05 µg/mL). All of extracts exhibited excellent antioxidant activity. The highest antioxidant activity at 2000 µg/mL was found in G.purpureum Vill. collected from Antalya (85.80 ± 0.26%). The radical scavenging activity of quercetin used as reference to compare in the antioxidant activity was found 85.51 ± 0.17 at 1000 µg/mL.Our findings revealed that Geranium and Erodium species screened in this study display high radical scavenging activity against DPPH and remarkable inhibitory activity against AChE.The phytochemical analysis of the active extracts to elucidate the compounds responsible for the mentioned activities is in progress in our laboratory