Flavopiridol Induces Proliferation of Adipose-derived Mesenchymal Stem Cells in a Dose-dependent Manner

Turantepe E., Kaçaroğlu D., Gürbüz N.

2. Uluslararası / 5. Ulusal Sağlık Hizmetleri Kongresi, Isparta, Türkiye, 2 - 04 Kasım 2023, ss.1-2

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Isparta
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.1-2
  • Lokman Hekim Üniversitesi Adresli: Evet

Özet

Introduction: Flavopiridol is a semi-synthetic flavonoid synthesized with inspiration from the

chromone alkaloid called Rohitukine. As a CDK inhibitor, it leads to the cell cycle arrest in the

G1/S and G2/M phases. Recent studies have revealed that flavonoids have the ability to

modulate the proliferation, self-renewal and differentiation potential of mesenchymal stem

cells. However, there are very few studies on stem cells with flavonoids and their mechanistic

effects.

Aim: In this study, we aimed to investigate the potential proliferative and protective effects of

flavopiridol in adipose-derived mesenchymal stem cells (AD-MSCs).

Methods: Adipose tissue obtained from a healthy female who underwent abdominal

liposuction surgery was used. Mesenchymal stem cells were isolated with collagenase from the

lipoaspirate waste of patients. After the cells were cultured, specific positive antibody, negative

marker cocktail and isotype control antibody were used for characterization. The potential


proliferative and protective effects of flavopiridol on MSC were detected by MTS assay. AD-

MSCs cells were seeded in 96-well plates and treated with different doses of flavopiridol, as


50, 100, 150, 200, 250 and 300 nM, for 48h. The results were evaluated by comparing to non-

treated (NT) condition.


Results: The MTS results show that the effect of flavopiridol depends on its concentration. The

cell viability of AD-MSCs was not affected at the 50 nM dose of flavopiridol. However, it

decreased by 10% after exposure to 100 nM and by 27% after exposure to 150 nM compared

to NT group. At doses of 200, 250, and 300 nM, flavopiridol did not reduce cell viability below

62%.

Discussion and Conclusion: This is the first to investigate the proliferative effect of

flavopiridol in AD-MSCs. Our study showed that flavopiridol has a dose-dependent

proliferative effect on AD-MSCs. Further studies are needed to investigate the mechanism of

action of flavopiridol in AD-MSCs and to determine its potential therapeutic applications.

Keywords: Flavopiridol, mesenchymal stem cell, AD-MSCs, cell proliferation