Akademik Veri Yönetim Sistemi
Journal of the Serbian Chemical Society - JSCS, cilt.1, sa.1, ss.1-5, 2024 (Hakemli Dergi)
Simple, accurate and sensitive spectrophotometric, chemometric and chromatographic methods were used for the simultaneous determination of emtricitabine (ETC) and tenofovir disoproxil fumarate (TDF) in tablets. In 1st derivative spectrophotometry, first derivative spectra of the solution of ETC and TDF in water were recorded as ∆λ = 4 nm and first derivative absorbances were measured at the zero-crossing points at 297.3 nm and 281.2 nm for ETC and TDF respectively. In ratio 1st derivative spectrophotometry measurements were recorded at 239.0 nm and 270.2 nm for ETC and TDF respectively. Then analytical signals were measured at the wavelengths corresponding to either maximum or minimum for both drugs. For these spectrophotometric methods Beer’s law is obeyed in the concentration range of 2-15 µg mL-1 for both drugs. As chemometric method, PLS technique was used. In chromatographic method, the separation was achieved on a C18 column with DAD (262 nm) and isocratic elution of methanol, acetonitrile and 0.1% orthophosphoric acid in the ratio of 40:40:20 % (v/v/v) respectively, containing mobile phase. Mean recovery and relative standard deviation of the methods were found as 97.51-100.17 % and 0.55 - 1.26 % respectively. All these methods were statistically compared, and they were successfully applied to a pharmaceutical preparation.